Associate Professor, Dr. YongXiang Chen

Address: New Life Science Building 332, Tsinghua University, Beijing 100084

Email: chen-yx@mail.tsinghua.edu.cn

Tel: +86-10-62772259

 

 

 

Education:

2002 - 2007    Ph.D. in Chemistry,   Department of Chemistry, Tsinghua University 

1998 - 2002    Bachelor in Chemistry, Department of Chemistry, Hunan University   

 

Working Experience:

2011 -            Associate Professor,  Department of Chemistry, Tsinghua University

2007 - 2011   Max-Planck & Humboldt Postdoctoral fellow, Max-Planck Institutfür

Molekulare Physiologie, Abteilung Chemische Biologie, Germany

 

Research Interests:

Protein and peptide chemistry; elucidating the regulating mechanism of protein posttranslational modifications on proteins’ function, localization and trafficking; developing methods for protein immobilization and labeling

 

Selected Publications:

1.    Zhang SY, Sperlich B, Li FY, Al-Ayoubi S, Chen HX, Zhao YF, Li YM, Weise K, Winter R*, Chen YX* Phosphorylation Weakens but Does Not Inhibit Membrane Binding and Clustering of K-Ras4B  ACS Chem. Biol., 2017, in press, DOI: 10.1021/acschembio.7b00165  (* corresponding author)

2.    Shi L, Chen H, Zhang SY, Chu TT, Zhao YF, Chen YX*, Li YM* Semi-synthesis of murine prion protein by native chemical ligation and chemical activation for preparation of polypeptide-α-thioester J. Pept. Sci., 2017, in press, DOI: 10.1002/psc.3008

3.    Chu TT, Gao N, Li QQ, Chen PG, Yang XF, Chen YX*, Zhao YF, Li YM* Specific knockdown of endogenous tau protein by peptide-directed ubiquitin-proteasome degradation Cell Chem. Biol., 2016, 23, 453-461 (front cover)

4.    Li L, Zhang SY, Li YM, Chen YX*, Dual-Labeling of Ubiquitin Protein by Chemoselective Reactions for Sensing UCH-L3. Mol. BioSyst. 2016, 12, 1764-1467

5.    He YH, Li YM, Chen YX* Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor, Talanta, 2016, 150, 340-345

6.    Chu TT, Li QQ, Qiu T, Sun ZY, Hu ZW, Chen YX*, Zhao YF, Li YM* Clearance intracellular high level of Tau protein directed by artificial synthetic hydrolase Mol. BioSyst., 2014, 10, 3081–3085 (back cover)

7.    Zhao L, Hu ZW, Tong P, Chen YX*, Zhao YF, Li YM* “Addition of artificial salt bridge by Ile646Lys mutation in gp41 coiled-coil domain regulates six-helical bundle formation” Bioorg. Med. Chem. Lett., 2013, 23, 2727

8.    Yi L+. Chen YX+, Lin PC, Schroder H, Niemeyer CM, Wu YW, Goody RS, Triola G, Waldmann H*  Direct immobilization of oxyamine-modified proteins from cell lysates. Chem. Commun., 2012, 48, 10829-10831 (+co-first author)

9.      Chen YX, Triola G, Waldmann H, Bioorthogonal chemistry for site-specific labeling and surface immobilization of proteins, Acc. Chem. Res., 2011, 44, 762-773

10.   Ismail S+, Chen YX+, Rusinova A+, Chandra A+, Bierbaum M, Gremer L, Triola G, Waldmann H, Bastiaens P, Wittinghofer A*, Regulation of a GDI-like transport system for farnesylated cargo by Arl2/3-GTP, Nature. Chem. Biol., 2011, 7, 942-949

11.   Chen YX+, Koch S+, Uhlenbrock K+, Weise K, Das D, Gremer L, Brunsveld L, Wittinghofer A, Winter R, Triola G, Waldmann H*, Synthesis of the Rheb and K-Ras4B GTPases, Angew. Chem. Int. Ed., 2010, 49, 6090-6095

12.   Chen YX, Zhao L, Huang ZP, Zhao YF, Li YM*, Facile synthesis of cyclopeptide -centered multivalent glycoclusters with “click chemistry” and molecular recognition study by surface plasmon resonance Bioorg. Med. Chem. Lett., 2009, 19, 3775-3778

13.   Chen YX, Zhao W, Zhao L, Zhao YF, Li YM*, Facile synthesis of a pentasaccharide mimic of a fragment of the capsular polysaccharide of Streptococcus pneumoniaetype 15C, Carbohydr. Res., 2008, 343, 607-614

14.   Chen YX, Du JT, Zhou LX, Liu XH, Zhao YF, Nakanishi H, Li YM*, Alternative O-GlcNAcylation and O-phosphorylation of Ser16 Inducing Different Conformational Disturbances to the N terminus of Murine Estrogen Receptor b, Chem. & Biol., 2006, 13, 937-944