Associate Professor, Dr. Yong-Xiang Chen  

Address: Life Science Building 332, Tsinghua University, Beijing 100084

Emailchen-yx@mail.tsinghua.edu.cn

Tel+86-10-62772259

ORCID: 0000-0003-3518-0139


Education:

2002 - 2007    Ph.D. in Chemistry, Department of Chemistry, Tsinghua University

1998 - 2002    Bachelor in Chemistry, Department of Chemistry, Hunan University


Working Experience

2016 -     PI, Associate Professor, Department of Chemistry, Tsinghua University

2011 - 2016   Associate Professor, Department of Chemistry, Tsinghua University

2007 - 2011   Max-Planck & Humboldt Postdoctoral fellow, Max-Planck Institut für

Molekulare Physiologie, Abteilung Chemische Biologie, Germany


Academic service

2022-  Editorial Board, Journal of Peptide Science  (Wiley)

2019 -  Editorial Board, Bioorganic Chemistry  (Elsevier)

Research Interests

Protein and peptide chemistry; elucidating the regulating mechanism of protein posttranslational modifications on proteins’ function, localization and trafficking.


Selected Publications:

1. Chang, R.; Chen, J.-L.; Zhang, G.-Y.; Li, Y.; Duan, H.-Z.; Luo, S.-Z.; Chen, Y.-X.*, Intrinsically Disordered Protein Condensate Modified Surface for Mitigation of Biofouling and Foreign Body Response. J. Am. Chem. Soc. 2022, online (DOI: 10.1021/jacs.2c02677) (*corresponding author)

2. Chang, R.#; Liu, Y.-J. #; Zhang, Y.-L.; Zhang, S.-Y.; Han, B.-B.; Chen, F.*; Chen, Y.-X.*, Phosphorylated and Phosphonated Low-Complexity Protein Segments for Biomimetic Mineralization and Repair of Tooth Enamel. Adv. Sci. 2022, 2103829

3. Wu, J.-J.#; Chen, F.-Y.#; Han, B.-B. #; Zhang, H.-Q. #; Zhao, L.; Zhang, Z.-R.; Li, J.-J.; Zhang, B.-D.; Zhang, Y.-N.; Yue, Y.-X.; Hu, H.-G.; Li, W.-H.; Zhang, B.*; Chen, Y.-X.*; Guo, D.-S.*; Li, Y.-M.*, CASTING: A Potent Supramolecular Strategy to Cytosolically Deliver STING Agonist for Cancer Immunotherapy and SARS-CoV-2 Vaccination CCS Chem. 2022, online (DOI: 10.31635/ccschem.022.202201859)

4. Hu, J.; Sun X.-M.; Su J.-Y.; Zhao Y.-F.; Chen Y.-X.*, Different Phosphorylation and Farnesylation Patterns Tune Rnd3-14-3-3 Interaction in Distinct Mechanisms, Chem. Sci. 2021, 12, 4432-4442.

5. Zhu, P.-C.; Chen, Y.-X.*, Facile Synthesis of Boc-Protected Selenocystine and its Compatibility with Late-Stage Farnesylation at Cysteine Site. Prot. Pep. Lett. 2021, 28 (6), 603-611.

6. Li, F.-Y.; Zhang, Z.-F.; Voss, S.; Wu, Y.-W.; Zhao, Y.-F.; Li, Y.-M.; Chen, Y.-X.*, Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide. Chem. Sci. 2020, 11 (3), 826-832.

7. Duan H.Z.; Nie Z.K.; Li Y., Chen Y.-X.*, Unremitting progresses for phosphoprotein synthesis. Curr. Opin. Chem. Biol. 2020, 58, 96-111.

8. Hackenberger, C. P. R.; Dawson, P. E.; Chen, Y.-X.; Hojo, H., Modern Peptide and Protein Chemistry: Reaching New Heights. J. Org. Chem. 2020, 85 (3), 1328-1330. (Editorial)

9. Han, B.-B.; Pan, Y.-C.; Li, Y.-M.; Guo, D.-S.*; Chen, Y.-X.* A host−guest ATP responsive strategy for intracellular delivery of phosphopeptides. Chem. Commun. 2020, 56, 5512-5515

10. Zhang, Y.-L.; Chang, R.; Duan, H.-Z.; Chen, Y.-X.*, Metal ion and light sequentially induced sol–gel–sol transition of a responsive peptide-hydrogel, Soft Matter, 2020, 16, 7652-7658.

11. Gao, N.; Huang, Y.-P.; Chu, T.-T.; Li, Q.-Q.; Zhou, B.; Chen, Y.-X.*; Zhao, Y.-F.; Li, Y.-M.*, TDP-43 specific reduction induced by Di-hydrophobic tags conjugated peptides. Bioorg. Chem. 2019, 84, 254-259.

12. Duan, H.-Z.; Chen, H.-X.; Yu, Q.; Hu, J.; Li, Y.-M.; Chen, Y.-X.*, Stereoselective synthesis of a phosphonate pThr mimetic via palladium-catalyzed gamma-C(sp(3))-H activation for peptide preparation. Org. & Biomol. Chem. 2019, 17 (8), 2099-2102.

13. Chen, H.-X.; Kang, J.; Chang, R.; Zhang, Y.-L.; Duan, H.-Z.; Li, Y.-M.; Chen, Y.-X.*, Synthesis of alpha,alpha-Difluorinated Phosphonate pSer/pThr Mimetics via Rhodium-Catalyzed Asymmetric Hydrogenation of beta-Difluorophosphonomethyl alpha-(Acylamino)acrylates. Org. Lett. 2018, 20 (11), 3278-3281.

14. Yu, Q.; Sun, J.; Huang, S.; Chang, H.; Bai, Q.; Chen, Y.-X.*; Liang, D.*, Inward Budding and Endocytosis of Membranes Regulated by de Novo Designed Peptides. Langmuir 2018, 34 (21), 6183-6193.

15. Hu, J.; Zhu, P.; Li, Y.; Chen, Y.-X.*, Synthesis of Ras proteins and their application in biofunctional studies. Chin. Chem. Lett. 2018, 29 (7), 1043-1050.

16. Huang, S.-Q.; Han, B.-B.; Li, Y.-M.; Chen, Y.-X.*, A site-specific branching poly-glutamate tag mediates intracellular protein delivery by cationic lipids. Biochem. Biophys. Res. Commun. 2018, 503 (2), 671-676.

17. Zhang, S.-Y.; Sperlich, B.; Li, F.-Y.; Al-Ayoubi, S.; Chen, H.-X.; Zhao, Y.-F.; Li, Y.-M.; Weise, K.; Winter, R.*; Chen, Y.-X.*, Phosphorylation Weakens but Does Not Inhibit Membrane Binding and Clustering of K-Ras4B. ACS Chem. Biol. 2017, 12 (6), 1703-1710.

18. Gao, N.; Chu, T. T.; Li, Q. Q.; Lim, Y. J.; Qiu, T.; Ma, M. R.; Hu, Z. W.; Yang, X. F.; Chen, Y.-X.*; Zhao, Y. F.; Li, Y. M.*, Hydrophobic tagging-mediated degradation of Alzheimer's disease related Tau. RSC Adv. 2017, 7 (64), 40362-40366.

19. Shi, L.; Chen, H.; Zhang, S.-Y.; Chu, T.-T.; Zhao, Y.-F.; Chen, Y.-X.*; Li, Y.-M.*, Semi-synthesis of murine prion protein by native chemical ligation and chemical activation for preparation of polypeptide--thioester. J. Pept. Sci. 2017, 23 (6), 438-444.

20. Kang, J.; Chen, H.-X.; Huang, S.-Q.; Zhang, Y.-L.; Li, F.-Y.; Li, Y.-M.; Chen, Y.-X.*, Facile synthesis of Fmoc-protected phosphonate pSer mimetic and its application in assembling a substrate peptide of 14-3-3 zeta. Tetrahedron Lett. 2017, 58 (26), 2551-2553.

21. Chu, T.-T.; Gao, N.; Li, Q.-Q.; Chen, P.-G.; Yang, X.-F.; Chen, Y.-X.*; Zhao, Y.-F.; Li, Y.-M.*, Specific Knockdown of Endogenous Tau Protein by Peptide-Directed Ubiquitin-Proteasome Degradation. Cell Chem. Biol. 2016, 23 (4), 453-461.

22. Li, L.; Zhang, S.-Y.; Li, Y.-M.; Chen, Y.-X.*, Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3. Mol. Biosystems 2016, 12 (6), 1764-1767.

23. He, Y.-H.; Li, Y.-M.; Chen, Y.-X.*, Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor. Talanta 2016, 150, 340-345.

24. Chu, T.-T.; Li, Q.-Q.; Qiu, T.; Sun, Z.-Y.; Hu, Z.-W.; Chen, Y.-X.*; Zhao, Y.-F.; Li, Y.-M.*, Clearance of the intracellular high level of the Tau protein directed by an artificial synthetic hydrolase. Mol. Biosystems 2014, 10 (12), 3081-3085.

25. Zhao, L.; Hu, Z. W.; Tong, P.; Chen, Y.-X.*; Zhao, Y. F.; Li, Y. M.*, Addition of artificial salt bridge by Ile646Lys mutation in gp41 coiled-coil domain regulates 6-helical bundle formation. Bioorg. Med. Chem. Lett. 2013, 23 (9), 2727-2732.

26. Yi, L.#; Chen, Y. -X.#; Lin, P. -C.; Schroder, H.; Niemeyer, C. M.; Wu, Y. W.; Goody, R. S.; Triola, G.; Waldmann, H.*, Direct immobilization of oxyamine-modified proteins from cell lysates. Chem. Commun. 2012, 48 (88), 10829-10831. (# co-first author)

27. Ismail, S. A.; Chen, Y. -X. ; Miertzschke, M. ; Vetter, I. R. ; Koerner, C.; Wittinghofer, A.*, Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119. EMBO J. 2012, 31 (20), 4085-4094.

28. Chen, Y. -X.; Triola, G.; Waldmann, H.*, Bioorthogonal Chemistry for Site-Specific Labeling and Surface Immobilization of Proteins. Acc. Chem. Res. 2011, 44 (9), 762-773.

29. Ismail, S. A. #; Chen, Y. -X. # ; Rusinova, A. #; Chandra, A. #; Bierbaum, M.; Gremer, L.; Triola, G.; Waldmann, H.; Bastiaens, P. I. H.; Wittinghofer, A. *: Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo. Nature Chemical Biology 2011, 7, 942-949.

30. Chen, Y. -X.#; Koch, S. #; Uhlenbrock, K. #; Weise, K.; Das, D.; Gremer, L.; Brunsveld, L.; Wittinghofer, A.; Winter, R.; Triola, G.; Waldmann, H.*, Synthesis of the Rheb and K-Ras4B GTPases. Angew. Chem. Int. Ed. 2010, 49 (35), 6090-6095.