Associate Professor, Dr. Yong-Xiang Chen
Address: Life Science Building 332, Tsinghua University, Beijing 100084
Email：chen-yx@mail.tsinghua.edu.cn
Tel：+86-10-62772259
Web of Science ResearcherID: D-4792-2017
https://www.webofscience.com/wos/author/record/D-4792-2017
ORCID: 0000-0003-3518-0139
Group Website: www.chenyx.group
Education:
2002 - 2007 Ph.D. in Chemistry, Department of Chemistry, Tsinghua University
1998 - 2002 Bachelor in Chemistry, Department of Chemistry, Hunan University
Working Experience：
2016 - PI, Associate Professor, Department of Chemistry, Tsinghua University
2011 - 2016 Associate Professor, Department of Chemistry, Tsinghua University
2007 - 2011 Max-Planck & Humboldt Postdoctoral fellow, Max-Planck Institut für
Molekulare Physiologie, Abteilung Chemische Biologie, Germany
Research Interests：
Developing synthetic tools toward protein and peptide with various modifications; Mechanistic elucidation of multi-modifications on protein’s activity in biological events; Biomedical application of protein and peptide.
Academic service：
2019- Editorial Board Member, Bioorganic Chemistry (Elsevier)
2022- Editorial Board Member, Journal of Peptide Science (Wiley)
2019- Member of the Professional Committee of Peptide, China Biochemical Pharmaceutical Industry Association
2023- Member of Scientific Program Committee, International Chemical Biology Society (ICBS)
Teaching Activities:
• Organic Chemistry A2 (48 class hours/semester)
• Organic Chemistry H1 Seminar (8 class hours/semester)
• Chemical Biology (48 class hours/semester)
Honors and Awards:
• “Design Star” Award, the 4th National Competition of Blended Teaching Innovative Design in Chinese Colleges and Universities (2022)
• The 1st prize in the 9th Teaching Contest for Young Teachers in Tsinghua University (2020)
• Honored with the element “phosphorus” on Periodic Table of Chinese Younger Chemists (2019)
• Honored with “Rising Star” in the 8th Chemical Protein Synthesis Meeting (2019, Berlin)
• Peptide Application Special Awards, the 15th Chinese International Peptide Symposium (2018, Shenzhen)
Publications:(in inverse chronological order, since 2016-present, as corresponding author *)
1. Liu, D.; Liu, Y.; Duan, H.-Z.; Chen, X.; Wang, Y.; Wang, T.; Yu, Q.; Chen, Y.-X.*; Lu, Y.*, Customized synthesis of phosphoprotein bearing phosphoserine or its nonhydrolyzable analog. Synthetic and Systems Biotechnology 2023, 8 (1), 69-78.
2. Duan, H.-Z.; Hu, C.; Li, Y.-L.; Wang, S.-H.; Xia, Y.; Liu, X.*; Wang, J.*; Chen, Y.-X.*, Genetically Encoded Phosphine Ligand for Metalloprotein Design. Journal of the American Chemical Society 2022, 14 (50), 22831-22837.
3. Chang, R.; Chen, J.-L.; Zhang, G.-Y.; Li, Y.; Duan, H.-Z.; Luo, S.-Z.; Chen, Y.-X.*, Intrinsically Disordered Protein Condensate-Modified Surface for Mitigation of Biofouling and Foreign Body Response. Journal of the American Chemical Society 2022, 144 (27), 12147-12157.
4. Chang, R.; Liu, Y.-J.; Zhang, Y.-L.; Zhang, S.-Y.; Han, B.-B.; Chen, F.*; Chen, Y.-X.*, Phosphorylated and Phosphonated Low-Complexity Protein Segments for Biomimetic Mineralization and Repair of Tooth Enamel. Advanced Science 2022, 9(6), 2103829.
5. Wu, J.-J.; Chen, F.-Y.; Han, B.-B.; Zhang, H.-Q.; Zhao, L.; Zhang, Z.-R.; Li, J.-J.; Zhang, B.-D.; Zhang, Y.-N.; Yue, Y.-X.; Hu, H.-G.; Li, W.-H.; Zhang, B.*; Chen, Y.-X.*; Guo, D.-S.*; Li, Y.-M.*, CASTING: A Potent Supramolecular Strategy to Cytosolically Deliver STING Agonist for Cancer Immunotherapy and SARS-CoV-2 Vaccination. CCS Chemistry 2022, DOI: 10.31635/ccschem.022.202201859.
6. Li, Y.; Chang, R.; Chen, Y.-X.*, Recent Advances in Post-polymerization Modifications on Polypeptides: Synthesis and Applications. Chemistry-an Asian Journal 2022, 17(14), e202200318.
7. Hu, J.; Sun, X.-M.; Su, J.-Y.; Zhao, Y.-F.; Chen, Y.-X.*, Different phosphorylation and farnesylation patterns tune Rnd3-14-3-3 interaction in distinct mechanisms. Chemical Science 2021, 12 (12), 4432-4442.
8. Zhu, P.-C.; Chen, Y.-X.*, Facile Synthesis of Boc-Protected Selenocystine and its Compatibility with Late-Stage Farnesylation at Cysteine Site. Protein and Peptide Letters 2021, 28 (6), 603-611.
9. Li, F.-Y.; Zhang, Z.-F.; Voss, S.; Wu, Y.-W.; Zhao, Y.-F.; Li, Y.-M.; Chen, Y.-X.*, Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide. Chemical Science 2020, 11 (3), 826-832.
10. Duan, H.-Z.; Nie, Z.-K.; Li, Y.; Chen, Y.-X.*, Unremitting progresses for phosphoprotein synthesis. Current Opinion in Chemical Biology 2020, 58, 96-111.
11. Hackenberger, C. P. R.*; Dawson, P. E.*; Chen, Y.-X.*; Hojo, H.*, Modern Peptide and Protein Chemistry: Reaching New Heights. Journal of Organic Chemistry 2020, 85 (3), 1328-1330.
12. Han, B.-B.; Pan, Y.-C.; Li, Y.-M.; Guo, D.-S.*; Chen, Y.-X.*, A host-guest ATP responsive strategy for intracellular delivery of phosphopeptides. Chemical Communications 2020, 56 (41), 5512-5515.
13. Zhang, Y.-L.; Chang, R.; Duan, H.-Z.; Chen, Y.-X.*, Metal ion and light sequentially induced sol-gel-sol transition of a responsive peptide-hydrogel. Soft Matter 2020, 16 (33), 7652-7658.
14. Duan, H.-Z.; Chen, H.-X.; Yu, Q.; Hu, J.; Li, Y.-M.; Chen, Y.-X.*, Stereoselective synthesis of a phosphonate pThr mimetic via palladium-catalyzed gamma-C(sp(3))-H activation for peptide preparation. Organic & Biomolecular Chemistry 2019, 17 (8), 2099-2102.
15. Gao, N.; Huang, Y.-P.; Chu, T.-T.; Li, Q.-Q.; Zhou, B.; Chen, Y.-X.*; Zhao, Y.-F.; Li, Y.-M.*, TDP-43 specific reduction induced by Di-hydrophobic tags conjugated peptides. Bioorganic Chemistry 2019, 84, 254-259.
16. Chen, H.-X.; Kang, J.; Chang, R.; Zhang, Y.-L.; Duan, H.-Z.; Li, Y.-M.; Chen, Y.-X.*, Synthesis of alpha,alpha-Difluorinated Phosphonate pSer/pThr Mimetics via Rhodium-Catalyzed Asymmetric Hydrogenation of beta-Difluorophosphonomethyl alpha-(Acylamino)acrylates. Organic Letters 2018, 20 (11), 3278-3281.
17. Huang, S.-Q.; Han, B.-B.; Li, Y.-M.; Chen, Y.-X.*, A site-specific branching poly-glutamate tag mediates intracellular protein delivery by cationic lipids. Biochemical and Biophysical Research Communications 2018, 503 (2), 671-676.
18. Hu, J.; Zhu, P.; Li, Y.; Chen, Y.-X.*, Synthesis of Ras proteins and their application in biofunctional studies. Chinese Chemical Letters 2018, 29 (7), 1043-1050.
19. Yu, Q.; Sun, J.; Huang, S.; Chang, H.; Bai, Q.; Chen, Y.-X.*; Liang, D.*, Inward Budding and Endocytosis of Membranes Regulated by de Novo Designed Peptides. Langmuir 2018, 34 (21), 6183-6193.
20. Zhang, S.-Y.; Sperlich, B.; Li, F.-Y.; Al-Ayoubi, S.; Chen, H.-X.; Zhao, Y.-F.; Li, Y.-M.; Weise, K.; Winter, R.*; Chen, Y.-X.*, Phosphorylation Weakens but Does Not Inhibit Membrane Binding and Clustering of K-Ras4B. ACS Chemical Biology 2017, 12 (6), 1703-1710.
21. Kang, J.; Chen, H.-X.; Huang, S.-Q.; Zhang, Y.-L.; Li, F.-Y.; Li, Y.-M.; Chen, Y.-X.*, Facile synthesis of Fmoc-protected phosphonate pSer mimetic and its application in assembling a substrate peptide of 14-3-3 zeta. Tetrahedron Letters 2017, 58 (26), 2551-2553.
22. Gao, N.; Chu, T. T.; Li, Q. Q.; Lim, Y. J.; Qiu, T.; Ma, M. R.; Hu, Z. W.; Yang, X. F.; Chen, Y.-X.*; Zhao, Y. F.; Li, Y. M.*, Hydrophobic tagging-mediated degradation of Alzheimer's disease related Tau. RSC Advances 2017, 7 (64), 40362-40366.
23. Shi, L.; Chen, H.; Zhang, S. Y.; Chu, T. T.; Zhao, Y. F.; Chen, Y.-X.*; Li, Y. M.*, Semi-synthesis of murine prion protein by native chemical ligation and chemical activation for preparation of polypeptide--thioester. Journal of Peptide Science 2017, 23 (6), 438-444.
24. Chu, T.-T.; Gao, N.; Li, Q.-Q.; Chen, P.-G.; Yang, X.-F.; Chen, Y.-X.*; Zhao, Y.-F.; Li, Y.-M.*, Specific Knockdown of Endogenous Tau Protein by Peptide-Directed Ubiquitin-Proteasome Degradation. Cell Chemical Biology 2016, 23 (4), 453-461.
25. Li, L.; Zhang, S.-Y.; Li, Y.-M.; Chen, Y.-X.*, Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3. Molecular Biosystems 2016, 12 (6), 1764-1767.
26. He, Y.-H.; Li, Y.-M.; Chen, Y.-X.*, Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor. Talanta 2016, 150, 340-345.
Invited Talks:
• Young Scholar Forum on Chemical Biology, organized by Professional Committee of Chemical Biology, Chinese Chemical Society, Virtual (2023)
• The 14th Australian Peptide Conference, Surfers Paradise, Australia (online talk, 2022)
• The 2nd Postdoctoral Annual Academic Conference on Molecular Sciences, Beijing, China (2022)
• The 27th American Peptide Symposium, Whistler, Canada (not be able to attend due to pandemic, 2022)
• Pacifichem 2021— “Advancing Frontiers in Peptide and Protein Science with Nano-to-Macro Molecular Solutions, New Technologies in Polyamide Synthesis and Applications” symposium, Virtual (2021)
• The 16th Chinese International Peptide Symposium, Hefei, China (2020)
• The 11th Chinese National Conference on Chemical Biology, Guangzhou, China (2019)
• HKU Chemical Biology Symposium 2018, Hongkong, China (2018)
• The 7th Sino-German Frontiers of Chemistry Symposium, Munich, Germany (2018)
• The 15th Chinese International Peptide Symposium, Shenzhen, China (2018)
• The 11th National Conference on Phosphorus Chemistry and Chemical Engineering, Wuhan, China (2017)
• Asian Chemical Biology Initiative 2017 Ulaanbaatar Meeting, Ulaanbaatar, Mongolia (2017)
• CCS 11th National Symposium on Natural Organic Chemistry, Shanghai, China (2016)
