Xiang, Yu

Associate Professor Yu Xiang

Contact: Department of Chemistry, Tsinghua University, Beijing 100084, China

Office: Rm. 142 New Biological Building

Tel.: +86-10-62782759

Email: xiang-yu@mail.tsinghua.edu.cn


Dr. Yu Xiang got his B.S. in Chemistry (2004) from Tsinghua University, China, and Ph.D. in Chemistry (2009) under Prof. Aijun Tong in Tsinghua University. After the postdoctoral research (2009-2012) with Prof. Yi Lu at University of Illinois at Urbana-Champaign, Dr. Xiang has worked as an associate professor in Department of Chemistry at Tsinghua University since 2012. He is currently working on the sulfur chemistry for modified nucleic acids as tools in chemical biology, including (1) Sulfur chemistry for post-synthetic nucleic acid modification; (2) Chemically modified oligonucleotides for controlled gene editing; (3) in vitro selection of covalent oligonucleotide inhibitors of protein-protein interactions. He serves on the editorial board of the journal Biosensors.

Research Area:

Sulfur chemistry for modified nucleic acids as tools in chemical biology

Lab website: https://xiang-lab.com


Analytical Chemistry

Experiments in Chemical Biology

Representative Publications:

1. Shang, J. C.; He, L.; Wang, J. Y.; Tong, A. J.; Xiang, Y.*, In Situ Visualizing Nascent mRNA by DNA-Templated Oxidative Amination of 4-Thiouridine. Bioconjugate Chemistry, 2022, 33, 164-171.

2. Gu C. M.; Xiao, L.; Shang, J. C.; Xu, X.; He, L.; Xiang, Y.*, Chemical Synthesis of Stimuli-Responsive Guide RNA for Conditional Control of CRISPR-Cas9 Gene Editing. Chemical Science, 2021, 12, 9934-9945.

3. Xu, X.#; Xiao, L.#; Gu, C. M.; Shang, J. C.; Xiang, Y.*, Wavelength-Selective Activation of Photocaged DNAzymes in Live Cells. ACS Omega, 2021, 6, 13153–13160.

4.  Wang, J. Y.; Shang, J. C.; Qin, Z. C.; Tong, A. J.; Xiang, Y.*, Selective and Sensitive Fluorescence "Turn-On" Detection of 4-Thiouridine in Nucleic Acids via Oxidative Amination. Chemical Communications 2019, 55, 13096-13099.

5. Xiao, L.#; Gu, C. M.#; Xiang, Y.*, Orthogonal Activation of RNA-Cleaving DNAzymes in Live Cells by Reactive Oxygen Species. Angewandte Chemie-International Edition 2019, 58, 14167-14172.

6. Feng, M. L.; Gu, C. M.; Sun, Y. P.; Zhang, S. Y.; Tong, A. J.; Xiang, Y.*, Enhancing Catalytic Activity of Uranyl-Dependent DNAzyme by Flexible Linker Insertion for More Sensitive Detection of Uranyl Ion. Analytical Chemistry 2019, 91, 6608-6615.

7. Feng, M. L.; Ruan, Z. Y.; Shang, J. C.; Xiao, L.; Tong, A. J.; Xiang, Y.*, Photocaged G-Quadruplex DNAzyme and Aptamer by Post-Synthetic Modification on Phosphodiester Backbone. Bioconjugte Chemistry 2017, 28, 549-555.

8. Xiao, L.; Zhou, Z. J.; Feng, M. L.; Tong, A. J.; Xiang, Y.*, Cationic Peptide Conjugation Enhances the Activity of Peroxidase-Mimicking DNAzymes. Bioconjugate Chemistry 2016, 27, 621-627.

9. Xiao, L.; Xiang, Y.*, Quantification of Total Phosphorothioate in Bacterial DNA by a Bromoimane-Based Fluorescence Method. Biotechnology Journal 2016, 11, 824-830.

10. Wang, X. Y.; Feng, M. L.; Xiao, L.; Tong, A. J.; Xiang, Y.*, Postsynthetic Modification of DNA Phosphodiester Backbone for Photocaged DNAzyme. ACS Chemical Biology 2016, 11, 444-451.